Detailed Locus Information for CNAG_04226T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | U4/U6.U5 tri-snRNP-associated protein 1 | |||||||||
| Gene Name | SNU66 | |||||||||
| TF Family Name | SART1 (Classification based on Fungal Transcription Factor DB) | |||||||||
| KEGG Linkout | KEGG::CNI03670  |
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| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 2,975 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 3,886 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 3,711 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 4,564 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 4,619 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 4,523 |
Gene Disruption Strategy
| Primer Name | Primer Description | Sequence |
|---|---|---|
| L1 | 5' flanking region primer 1 | GCCCTTTTTGGTATGACAG |
| L2 | 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTGTGAGGGATTCCTTGTTC |
| PO | Southern blot probe primer | ATACATCAGCCTGTCCTGC |
| R1 | 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGGTTGGAAACAAGCAGTCG |
| R2 | 3' flanking region primer 2 | TCAGAAGAATCAAGAGGTCG |
| SO | diagnostic screening primer, pairing with B79 | ACTTACATTGCCTTCCTCG |