Detailed Locus Information for CNAG_06327T1
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | DNA-binding protein creA | |||||||||
| Gene Name | ||||||||||
| TF Family Name | C2H2 zinc finger (Classification based on Fungal Transcription Factor DB) | |||||||||
| KEGG Linkout | KEGG::CNN00550  |
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| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 9,790 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 4,697 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 4,989 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 5,497 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 3,565 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 8,642 |
Gene Disruption Strategy
| Primer Name | Primer Description | Sequence |
|---|---|---|
| L1 | 5' flanking region primer 1 | GGAGAAGGGAAAGATGGGTG |
| L2 | 5' flanking region primer 2 | GCTCACTGGCCGTCGTTTTACTGTTTCCACTATTGGGAGAG |
| PO | Southern blot probe primer | ACGGGTTACGAGAAATGC |
| R1 | 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGAGGTGAAGGTGTGAGATTG |
| R2 | 3' flanking region primer 2 | CAATAATGTCCTCGCCAG |
| SO | diagnostic screening primer, pairing with B79 | GTATGAAGGGAAGGTGCTG |