Detailed Locus Information for CNAG_03527T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
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| Gene Annotation | cytoplasmic protein | |||||||||
| Gene Name | HEL2 | |||||||||
| TF Family Name | C2H2 zinc finger (Classification based on Fungal Transcription Factor DB) | |||||||||
| KEGG Linkout | KEGG::CNG00670  |
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| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 7,116 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 6,073 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 6,074 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 4,707 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 2,839 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 6,269 |
Gene Disruption Strategy
| Primer Name | Primer Description | Sequence |
|---|---|---|
| L1 | 5' flanking region primer 1 | CCATCTCACGGTTGATACG |
| L2 | 5' flanking region primer 2 | CTGGCCGTCGTTTTACTCAAACCCTTATCCCACCG |
| PO | Southern blot probe primer | CTATGAGCGACATTCACAGAC |
| R1 | 3' flanking region primer 1 | GTCATAGCTGTTTCCTGTCTTTCCCTGCTTTGCCTGC |
| R2 | 3' flanking region primer 2 | CGTCATCCGTCGTGTAACTG |
| SO | diagnostic screening primer, pairing with B79 | TCTCGCAAGGTCAGAGTTC |
Phenotype Assays
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| Mating Data | |
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| Virulence Factor Production | |
| Capsule | Urease |
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| Melanin | |
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| Stress Response and Adaptation | |
| Osmotic Stress | |
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| Oxidative Stress | Heavy Metal Stress |
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| Cell Wall Stress | |
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| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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